In order to improve work efficiency, simplify work flow and overcome weaknesses of conventional phase position calibration work, research on application of multi-rotor UAV in telemetry and control system in phase position calibration work has been done. The present situation that existed in the phase position calibration work of a integrated telemetry and control system has been analysed, as well as demands of the multi-rotor UAV application in the phase position calibration work. Math model of the phase position calibration work by using multi-rotor UAV has been established. In the end, the multi-rotor UAV type selection work has been done and a mini new beacon transmitter has been designed and manufactured. The mounting frame which fixed the beacon transmitter and flying battery had been designed and manufactured. The production of this research has been proofed by practical application, while the multi-rotor UAV can be applied in the phase position calibration work, it can also be used to testing telemetry and control system’s tracking performance, and it is endowed with practical application and popularization value to the similar telemetry and control systems.