A self-adaptive communication protocol was applied to a certain telecommunication satellite, the telemetry parameter capacity and storage location of remote terminal didn’t need to be pre-established in the bus control terminal. When on power, the information and bus status of remote terminal was transferred to bus control terminal dynamically, solving the problem of bad self-adaptive and poor universality of integrated electronic systems. In order to verify the communication function of self-adaptive 1553B bus based on certain telecommunication satellite, an accurate and efficient automatic testing system was proposed, and a bus monitor based on self-adaptive 1553B bus was applied. The results showed that compared with traditional communication protocol of 1553B bus, the proposed testing method validated the communication function based on self-adaptive 1553B bus was real-time, robust and efficient.